genetic diversity in four microsatellite loci bms1915, bms1350, lgb and ilsts45 in baluchi sheep

the poly morphism of 4 micro satellite loci (bms1350, lgb, ilsts45, bms1915) associated with functional traits were evaluated for detecting the diversity level in baluchi breed of sheep. one hundred eighty five blood samples were collected from sheep herd in abbas-abad breeding station. dna extraction was performed using guanidinethiocyanate silica gel method. gene fragments of micro satellites were amplified by polymerase chain reaction based on the recommended standard method. the pcr products were visible using poly acry lamide gel electrophoresis and stain methods with silver. alleles were typed using the photocapt software version 3. all loci were in the hardy-weinberg equilibrium (p<0.005). e, d, b and a alleles had the highest frequency level in bms1915(0.53),bms1350(0.71), lgb(0.78 ) and ilsts45(0.91) respectively . heterozygosity range varied between 0.91 in bms1350 locus and 0.83 in lgb locus. the estimated highest value for bms1350 locus of shannon index was 2.05. the lowest estimated value for lgb locus was 1.65. the results demonstrated that there was a high genetic diversity in of baluchi sheep population despite running the official breeding programs.